Coronary Artery Smooth Muscle Cells

Application

vascular research, supply of blood to heart muscle

Cell Line Origin

Artery

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

General description

PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Preparation Note

2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PCASMC Culture Protocol.